pbabe puro gfp lamin a plasmid (Addgene inc)
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pbabe puro gfp lamin a plasmid
Pbabe Puro Gfp Lamin A Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+gfp/pBABE-puro-GFP-wt-lamin+A+(Plasmid+%2317662)/pmc12863304-204-12-16
Average 93 stars, based on 61 article reviews
Pbabe Puro Gfp Lamin A Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbabe+gfp/pBABE-puro-GFP-wt-lamin+A+(Plasmid+%2317662)/pmc12863304-204-12-16
Average 93 stars, based on 61 article reviews
pbabe puro gfp lamin a plasmid - by Bioz Stars,
2026-09
93/100 stars
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Clone Assay:Article Title: Genome-wide CRISPR screens in spheroid culture reveal that the tumor suppressor LKB1 inhibits growth via the PIKFYVE lipid kinase Article Snippet: The alleles also contain mutations that confer resistance to CRISPR/Cas9 editing (without changing the amino acid sequence) with all the sgRNAs against LKB1 in the TKOv3 CRISPR library (Addgene 125517, a gift from Jason Moffat). .. These alleles were then cloned into Article Title: Genome-wide CRISPR screens in spheroid culture reveal that the tumor suppressor LKB1 inhibits growth via the PIKFYVE lipid kinase. Article Snippet: The alleles also contain mutations that confer resistance to CRISPR/ Cas9 editing (without changing the amino acid sequence) with all the sgRNAs against LKB1 in the TKOv3 CRISPR library (Addgene 125517, a gift from Jason Moffat). .. These alleles were then cloned into Construct:Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation. Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAVGFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAV-GFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Transfection:Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation. Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAVGFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAV-GFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Plasmid Preparation:Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation. Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAVGFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Article Title: CDK9 interacts with a RanGTP-NEMP1-Importin β complex to regulate erythroid enucleation Article Snippet: .. Antibodies, inhibitors and other reagents are listed in supplemental Table 1. pBABE-Flag-Cdk9-IRES-eGFP, pBABE-Flag-Cdk9-T186A-IRES-eGFP and pBABE-Flag-Cdk9-D167N-IRES-eGFP were gifts from Andrew Rice (Addgene plasmid #28096, RRID: Addgene_28096; Addgene plasmid #28097, RRID: Addgene_28097; Addgene plasmid #28098, RRID: Addgene_28098). Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAV-GFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Article Title: PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer. Article Snippet: All cell lines were tested to be mycoplasma-free using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma Aldrich). .. All shRNAs targeting PPP2R2A were purchased from Sigma-Aldrich. shPPP2R2A-1, TRCN0000002490; shPPP2R2A-2, TRCN0000002491; shPPP2R2A-5, TRCN0000002493. pMIG FLAG-PPP2R2A was obtained from Addgene (Plasmid #3804), and pBabe GFP-PPP2R2A was generated by PCR amplification of full-length PPP2R2A from pMIG FLAGPPP2R2A and its subsequent subcloning into Article Title: PCSK5 M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells Article Snippet: Cells were confirmed negative for mycoplasma (R&D Systems, CUL001B) in May 2023 and were used within one month of thawing for all experiments. .. GDF11 secretion assay—pLX302 GDF11-V5 puro (RRID:Addgene_83097), pLX304 (wildtype) PCSK5-V5 blast (RRID:Addgene_83100), and pLX304 PCSK5 (T288P)-V5 blast (RRID:Addgene_83101) were described previously ( 28 ). Expressing:Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation. Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAVGFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Article Title: The V-ATPase complex component RNAseK is required for lysosomal hydrolase delivery and autophagosome degradation Article Snippet: For in vivo interscapular brown adipose tissue (iBAT) targeting, pAAV-GFP (Cell Biolabs; #AAV-400) was used as a transduction control. .. For endogenous tagging of Rnasek at the C-terminal end with a MYC tag (RNAseK-MYC) in MEF cells, the following constructs were transfected into cells: pcDNA3.3-hCas9 (a gift from George Church, Addgene plasmid #41815), Generated:Article Title: PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer. Article Snippet: All cell lines were tested to be mycoplasma-free using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma Aldrich). .. All shRNAs targeting PPP2R2A were purchased from Sigma-Aldrich. shPPP2R2A-1, TRCN0000002490; shPPP2R2A-2, TRCN0000002491; shPPP2R2A-5, TRCN0000002493. pMIG FLAG-PPP2R2A was obtained from Addgene (Plasmid #3804), and pBabe GFP-PPP2R2A was generated by PCR amplification of full-length PPP2R2A from pMIG FLAGPPP2R2A and its subsequent subcloning into Polymerase Chain Reaction:Article Title: PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer. Article Snippet: All cell lines were tested to be mycoplasma-free using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma Aldrich). .. All shRNAs targeting PPP2R2A were purchased from Sigma-Aldrich. shPPP2R2A-1, TRCN0000002490; shPPP2R2A-2, TRCN0000002491; shPPP2R2A-5, TRCN0000002493. pMIG FLAG-PPP2R2A was obtained from Addgene (Plasmid #3804), and pBabe GFP-PPP2R2A was generated by PCR amplification of full-length PPP2R2A from pMIG FLAGPPP2R2A and its subsequent subcloning into Amplification:Article Title: PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer. Article Snippet: All cell lines were tested to be mycoplasma-free using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma Aldrich). .. All shRNAs targeting PPP2R2A were purchased from Sigma-Aldrich. shPPP2R2A-1, TRCN0000002490; shPPP2R2A-2, TRCN0000002491; shPPP2R2A-5, TRCN0000002493. pMIG FLAG-PPP2R2A was obtained from Addgene (Plasmid #3804), and pBabe GFP-PPP2R2A was generated by PCR amplification of full-length PPP2R2A from pMIG FLAGPPP2R2A and its subsequent subcloning into Subcloning:Article Title: PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer. Article Snippet: All cell lines were tested to be mycoplasma-free using the LookOut Mycoplasma PCR Detection Kit (MP0035, Sigma Aldrich). .. All shRNAs targeting PPP2R2A were purchased from Sigma-Aldrich. shPPP2R2A-1, TRCN0000002490; shPPP2R2A-2, TRCN0000002491; shPPP2R2A-5, TRCN0000002493. pMIG FLAG-PPP2R2A was obtained from Addgene (Plasmid #3804), and pBabe GFP-PPP2R2A was generated by PCR amplification of full-length PPP2R2A from pMIG FLAGPPP2R2A and its subsequent subcloning into Mutagenesis:Article Title: PCSK5 M452I is a recessive hypomorph exclusive to MCF10DCIS.com cells Article Snippet: Cells were confirmed negative for mycoplasma (R&D Systems, CUL001B) in May 2023 and were used within one month of thawing for all experiments. .. GDF11 secretion assay—pLX302 GDF11-V5 puro (RRID:Addgene_83097), pLX304 (wildtype) PCSK5-V5 blast (RRID:Addgene_83100), and pLX304 PCSK5 (T288P)-V5 blast (RRID:Addgene_83101) were described previously ( 28 ). |
